English translation for "reverse phase hplc"
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- 反相高压液相层析
Related Translations:
hplc: 分离高效液相色谱法高性能液体色谱高压液相层析
- Example Sentences:
| 1. | The method for the determination of amino acid neurotransmitters in brain by reverse phase hplc system with the precolumn phthali aldehyde derivatization
柱前衍生反相高效液相色谱法测定脑组织中氨基酸类神经递质 | | 2. | It is introduced that the quantitative analysis of trichlorfon by reverse phase hplc , using acetonitrile and water ( 50 : 50 ) as mobile phase , using cn column and ultraviolet detector
摘要本文采用即以乙腈+水( 50 + 50 )为流动相，用cn柱和紫外检测器分离测定敌百虫原药及40 %敌?毒乳油中敌百虫反相高效液相色谱法。 | | 3. | The quantitative analysis of 25 % chlorpyrifos and triazophos ec by reverse phase hplc was introduced , using acetonitrile and water ( 74 : 26 ) as mobile phase , using c18 column and ultraviolt detector
摘要本文叙述了采用反相高效液相色谱法即以乙腈+水为流动相， c18柱和紫外检测器同柱分离侧定25 %毒?唑乳油中的毒死蜱和三唑磷。 | | 4. | Imidacloprid and triazophos in mixture were determined simultareously by reverse phase hplc , methanol and acetonitrile and water and glacial acetic acid as mobile phase , c18 column and uv200 detecter
摘要采用c18不锈钢柱和uv200型检测器，以甲醇?乙腈?水的混合液为流动相，用反相高效液相色谱法对吡虫啉?三唑磷复配制剂进行定量分析测定。 | | 5. | The crude venom contains a mixture of compounds with different types of biological activities . two novel peptide neueotoxins were purified from the venom of chilobrachys jingzhao by using combination of ion - exchange and reverse phase hplc
海南敬钊缨毛蛛( chilobrachysjingzhao )毒素是最近在海南发现的蜘蛛新种，该蜘蛛粗毒中含有多种活性多肽和蛋白质成分。 | | 6. | Secondly , determination of the antimicrobial activities of synthetic hpab - . i ) the mature peptide of hpab was synthesized by fomc method and purified by reverse phase hplc . the molecular weight of synthesis peptide was 4334 . 96da characterized by mass spectrometry , highly agreed with the theoretical mw of hpab - 3
2 . hpab一p抗菌活性初步鉴定:采用固相法合成了hpab一p成熟肤，经即一hplc纯化后用质谱测定合成肤的分子量为4334 . % da ，与其理论分子量相符。 | | 7. | Both hainantoxin - iv ( hntx - iv ) and . hainantoxin - v ( hntx - v ) w ere peptide toxins isolated from the venom of the spider seleconosmia hainana by means of ion - exchange chromatograph and reverse phase hplc . hntx - iv , 3989 da , contained 35 amino acid residues with three disulfide bonds , while the molecular weight of hntx - v was 3972 da and had three disulfide bonds , too
海南捕鸟蛛毒素- ( hntx - )和海南捕鸟蛛毒素- ( hntx - )是从海南捕鸟蛛粗毒中经阳离子交换和反相hplc柱层析分离出来的两种神经毒素， maldi - tof质谱鉴定前者相对分子质量为3 ， 989da ，含有三对二硫键，由35个氨基酸残基组成；后者相对分子质量为3 ， 972da ，与前者只相差16个质子，也含有三对二硫键。 | | 8. | The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins
将带有hwtx -基因的ppic9k经blgii线性化后，转化酵母宿主菌gs115原生质体后经筛选阳性克隆并经表型鉴定为his ~ + mut ~ s酵母菌，进一步用遗传毒素g418筛选多拷贝的转化菌株，命名为gh1 ；将gh1甲醇酵母菌用0 . 5的甲醇诱导表达，发酵上清经90饱和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超滤膜超滤，再经cm阳离子交换， c _ ( 18 )反相hplc纯化得到分子量为4kd左右的组分，其中4289 . 05的组分经质谱鉴定，氨基酸组成分析和序列测定为正确的表达产物，生物学活性表明其活性为天然毒素活性70 % ，表达量为80mg / l 。 | | 9. | A neurotoxic peptide ( named huwentoxin - v ) was purified from the venom of the spider by a combination of ion exchange chromatography and reverse phase hplc . hwtx - v has 35 amino acid residues , with the molecular mass 4111 . 4da . the amino acid sequence has been determined as nh2 - ecrwylggcsqdgdcckhlqchsn - yewcvwdgtfs - cooh , which consists of 6 cys , formed three pairs of disulfide bridges
本文报道从虎纹捕鸟蛛( selenocoimahuwena )粗毒中，结合阳离子交换和反相高效液相色谱分离纯化出一种昆虫毒素，命名为虎纹捕鸟蛛毒素- ( huwentoxin - ， hwtx - ) ；其天然突变体( themutantofhuwentoxin ， mhwtx - )也同时纯化出来。 |
- Similar Words：
- "reverse period" English translation, "reverse peristalsis" English translation, "reverse phase" English translation, "reverse phase chromatography" English translation, "reverse phase evaporation" English translation, "reverse phase protection" English translation, "reverse phase reflex" English translation, "reverse phase relay" English translation, "reverse phase silica gel" English translation, "reverse phase thin layer chromatography" English translation
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